LOCUS BG083295 583 bp mRNA linear EST 12-MAY-2010 DEFINITION H3086F10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3086F10 5', mRNA sequence. ACCESSION BG083295 VERSION BG083295.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 583) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083295.1. Other_ESTs: H3086F10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3086 row: F column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..583 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3086F10-5" /db_xref="taxon:10090" /clone="H3086F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 158 a 147 c 137 g 141 t ORIGIN 1 agcatggtgg ctcacagcca tctgcagctc taggaccagg ggatctgaca ccctcttctg 61 tacttcatgg acactgggcc tacatgtaca cagacaaaca tgcaagcaaa acattcatac 121 aaagaaacca gcaccttgtc tccatcccga cttcagtgct gtcagtcccc aggtcacttg 181 ccagctcctg tgcggcacag tggggaccat ggcaagagac tgtttgtcgt caagccttct 241 ttatactatg acgctcgctt cttgaggtta atgaaattct accttatgtt gactgggatc 301 ccagttataa ttggcataac actggtgaac atatttattg gtgaagccga gcttgcagaa 361 atcccagaag gctacatccc tgaacactgg gagtattaca agcatccgat atcaagatgg 421 atcgcccgga atttctatga tggccctgaa aagaactacg agaaaaccct ggctatcctc 481 cagattgaat ctgaaaaggc tgagttacgg ttagaggagc aggaagttcg aaggctgatg 541 cgtgcccgag gtgatggacc ctggtatcaa tttccaaccc ctg //