LOCUS BG083274 596 bp mRNA linear EST 12-MAY-2010 DEFINITION H3086E01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3086E01 5', mRNA sequence. ACCESSION BG083274 VERSION BG083274.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 596) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083274.1. Other_ESTs: H3086E01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3086 row: E column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..596 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3086E01-5" /db_xref="taxon:10090" /clone="H3086E01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 217 a 150 c 90 g 139 t ORIGIN 1 taatctcagt cagtagatat gtgtattaac ttttaggcag ggtctgaaat tcgttaccat 61 gatcctgcct gctaccaaag aaattcctgc atattatcac tgacactgaa ttacagacaa 121 ccaaaacaaa acaaaaacat aaacaaaaga accaaaacaa acaaaacacc ctcaaaaaca 181 aaacagaaca aaaccaaaaa aaaaaaccca aacaaacaaa caaacaaaca aaaccctcaa 241 aaacaaaaac catgcctgct actcatacaa gataaaacag aaaggtacaa agtaaactat 301 gcaattatgc aagtggccag accgttatgg ctatggcaca gctatggcta taaccagggc 361 actcaaccct gttgtctcag gtagctgcag cactccgtat ttaacagcca tggtcaatga 421 aaggcaattc agtttccatg acaatttctc acttggatat ctgttcccat ggcctttgcg 481 ttcacagctt tatttgaggt gtattgggaa ttgaacccaa agcctgctac ccctgcacta 541 cccctgcagg cctgtgccta acgtttatgc catctcttta ctctccttct ttgctg //