LOCUS BG083202 649 bp mRNA linear EST 12-MAY-2010 DEFINITION H3085F07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3085F07 5', mRNA sequence. ACCESSION BG083202 VERSION BG083202.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 649) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083202.1. Other_ESTs: H3085F07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3085 row: F column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..649 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3085F07-5" /db_xref="taxon:10090" /clone="H3085F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 176 a 135 c 137 g 201 t ORIGIN 1 gcttactctt ttttagcaca gggaaaagaa acttgttacc actcaccctg tcagatgtgt 61 aattgaacaa agctgaatct ttcttctggg acccagagtt agagatcgtt cctttcctta 121 gcctactgaa tattggttgg caacattgga atttagtgga ctaattagtt cctctcatct 181 tacccagccc tttaagcccg ttcctcaact aagggtgtag ttactattct gggttcttaa 241 caaactgtgt gtcagcgttc tggtcacact tgatggtact gccttgagaa ccattgaaaa 301 agataaaatc atccctagtc atttagaaat atttgccttt ttcagggaca agtttgggag 361 caacagtggg gacaaaggtg gcaagttacg gagcctcaac aacttggtat ctgaacaaag 421 agggccgtgg agagctcagt ggtagagcac ttgcttagca tgcacaaggc tctaggttca 481 attcccagta ccatatacat aaaattttca tgtttaccct taaaaattaa ttacacttat 541 ttgttgttgg tctctgtatt atggcatacc ataaaccccg aggcctgata ttatttgatg 601 tcactcggac attgctgggt atcacaactc aggtgtcgag gtgtttcag //