LOCUS BG083201 721 bp mRNA linear EST 12-MAY-2010 DEFINITION H3085F06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3085F06 5', mRNA sequence. ACCESSION BG083201 VERSION BG083201.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 721) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083201.1. Other_ESTs: H3085F06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3085 row: F column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..721 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3085F06-5" /db_xref="taxon:10090" /clone="H3085F06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 207 a 170 c 197 g 147 t ORIGIN 1 acgtgttcct ctagtcgtac ttgccgggat tgatcttggc cgagatggag acgcaaagca 61 ccggaacgga ggacggcttt actcccgtca cgcacagagg gggccgccgg gcgaagaaac 121 gacaggctga gcagtcgtca gcggcgggac aggatggaga agccggacgc atggacacgg 181 aggaggcgcg gccggcgaag aggccggtct tcccgccgct ctccggggat cagctcctga 241 ctgggaaaga agaaacgagg aaaattcccg tcccaggtaa cagatacaca ccgttgaaag 301 aaaactggat gaagatattt actccgattg tagaacattt gggacttcag atacgcttta 361 acctgaaatc aaggaatgta gaaatcagga cttgtaaaga caccaaggat gtcagtgccc 421 tgacaaaagc ggccgacttt gtgaaagcct ttgttcttgg ctttcaggtg gaggatgcac 481 ttgcccttat ccgactagat gacctcttcc tagagtcttt tgaaataact gatgttaagc 541 ccctaaaagg agaccacctg tcaagggcaa taggaagaat tgctggcaaa ggaggaaaaa 601 ccaaatttac catagagaac gtaacacgga cacggattgt cctggcagat gtgcacgttc 661 acattcttgg ctctttccaa aatatcaaga tggcaagaac agctatttgc aacctcatcc 721 t //