LOCUS       BG083201                 721 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3085F06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3085F06 5', mRNA sequence.
ACCESSION   BG083201
VERSION     BG083201.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG083201.1.
            Other_ESTs: H3085F06-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3085  row: F  column: 06
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3085F06-5"
                     /db_xref="taxon:10090"
                     /clone="H3085F06"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          207 a          170 c          197 g          147 t
ORIGIN      
        1 acgtgttcct ctagtcgtac ttgccgggat tgatcttggc cgagatggag acgcaaagca
       61 ccggaacgga ggacggcttt actcccgtca cgcacagagg gggccgccgg gcgaagaaac
      121 gacaggctga gcagtcgtca gcggcgggac aggatggaga agccggacgc atggacacgg
      181 aggaggcgcg gccggcgaag aggccggtct tcccgccgct ctccggggat cagctcctga
      241 ctgggaaaga agaaacgagg aaaattcccg tcccaggtaa cagatacaca ccgttgaaag
      301 aaaactggat gaagatattt actccgattg tagaacattt gggacttcag atacgcttta
      361 acctgaaatc aaggaatgta gaaatcagga cttgtaaaga caccaaggat gtcagtgccc
      421 tgacaaaagc ggccgacttt gtgaaagcct ttgttcttgg ctttcaggtg gaggatgcac
      481 ttgcccttat ccgactagat gacctcttcc tagagtcttt tgaaataact gatgttaagc
      541 ccctaaaagg agaccacctg tcaagggcaa taggaagaat tgctggcaaa ggaggaaaaa
      601 ccaaatttac catagagaac gtaacacgga cacggattgt cctggcagat gtgcacgttc
      661 acattcttgg ctctttccaa aatatcaaga tggcaagaac agctatttgc aacctcatcc
      721 t
//