LOCUS BG082926 680 bp mRNA linear EST 12-MAY-2010 DEFINITION H3082D08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3082D08 5', mRNA sequence. ACCESSION BG082926 VERSION BG082926.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 680) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082926.1. Other_ESTs: H3082D08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3082 row: D column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..680 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3082D08-5" /db_xref="taxon:10090" /clone="H3082D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 180 c 203 g 143 t ORIGIN 1 gcctcttggg acaagacagt gcgtctgtgg gacatgttcg acagttggag aaccaaagag 61 accttgactc tgacctctga cgccctagct gtgactttcc ggcctgatgg tgcagagctg 121 gctgtggcca cgctgaattc acagatcacg ttttgggacc ctgagaatgc tgtacaggtg 181 ggctccatcg agggcaggca tgacctgaag accggcagga aggagctgga caagatcaca 241 gccaaacatt cagccaaggg caaggccttc actaccctgt gttactctgc ggacgggcag 301 agcatcctgg cgggaggcat gtccaagttc gtgtgccttt accacgtccg ggagcaaatc 361 ctggtgaaga ggtttgagct ctcctgcaac ctgtctctgg atgccatgga ggaattcctg 421 aaccggagaa agatgacaga gtttggcaac ctggcactca ttgatcaaga tgctggggag 481 gagaatgggg tgggcagttc cactgccagg agtaaggaaa ggtgatatga gctccagaca 541 cttcaaacct gaaatcaggg tgacttctct gcgcttctct cccacggggc gctgctgggc 601 ggccaccagc acagagggcc tcctcatctt ctccctggat gcccagatgc tcttcgaccc 661 atttgagctg gacaccagtg //