LOCUS BG082918 702 bp mRNA linear EST 12-MAY-2010 DEFINITION H3082C12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3082C12 5', mRNA sequence. ACCESSION BG082918 VERSION BG082918.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 702) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082918.1. Other_ESTs: H3082C12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3082 row: C column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..702 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3082C12-5" /db_xref="taxon:10090" /clone="H3082C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 181 c 177 g 162 t ORIGIN 1 gaagcatcct tgcttgggtt tgcagtcttc tgcggcaggc attctcggag gaaaccagcc 61 aaggactaac tacgaccatg agattggcag tgatttgctt ttgcctgttt ggcattgcct 121 cctccctccc ggtgaaagtg actgattctg gcagctcaga ggagaagctt tacagcctgc 181 acccagatcc tatagccaca tggctggtgc ctgacccatc tcagaagcag aatctccttg 241 cgccacagaa tgctgtgtcc tctgaagaaa aggatgactt taagcaagaa actcttccaa 301 gcaattccaa tgaaagccat gaccacatgg acgacgatga tgacgatgat gatgacgatg 361 gagaccatgc agagagcgag gattctgtgg actcggatga atctgacgaa tctcaccatt 421 cggatgagtc tgatgagacc gtcactgcta gtacacaagc agacactttc actccaatcg 481 tccctacagt cgatgtcccc aacggccgag gtgatagctt ggcttatgga ctgaggtcaa 541 agtctaggag tttccaggtt tctgatgaac agtatcctga tgccacagat gaggacctca 601 cctctcacat gaagagcggt gagtctaagg agtccctcga tgtcatccct gttgcccagc 661 ttctgagcat gccctctgat caggacaaca acggaaaggg ca //