LOCUS       BG082675                 667 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3078H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3078H10 5', mRNA sequence.
ACCESSION   BG082675
VERSION     BG082675.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG082675.1.
            Other_ESTs: H3078H10-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3078  row: H  column: 10
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3078H10-5"
                     /db_xref="taxon:10090"
                     /clone="H3078H10"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          162 a          187 c          173 g          145 t
ORIGIN      
        1 cgcctggctg ggagcagccg ccgcggaggt ctttggccag tatcgattcc acctgtccac
       61 aaacatgggg aatatctttg caaacctctt caagggcctt tttggcaaaa aagaaatgcg
      121 cattctcatg gtgggcctgg atgctgcagg gaagacaaca attctataca aacttaagct
      181 gggcgaaatt gtgaccacca ttcccaccat tggtttcaat gtggagactg ttgaatacaa
      241 gaatatcagc ttcaccgtgt gggatgtggg cggccaggac aagatccggc cgctgtggcg
      301 ccactacttc cagaacaccc aaggcttgat cttcgtagtg gacagcaatg acagagagcg
      361 tgtgaacgag gcccgtgaag agctcatgag gatgctagct gaagatgagc tccgagatgc
      421 tgttctcttg gtgtttgcca acaagcagga cctccccaat gccatgaatg cggccgaaat
      481 cacagacaag ctggggctgc actctctacg ccacaggaac tggtacattc aggccacctg
      541 tgccaccagc ggggacgggc tctatgaagg actagattgg ctgtctaatc agctccggaa
      601 ccagaagtga accagacccc tccctccccc tcacttcctc ttctccgccc tcagctttcc
      661 tctcatg
//