LOCUS BG082628 672 bp mRNA linear EST 12-MAY-2010 DEFINITION H3078C10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3078C10 5', mRNA sequence. ACCESSION BG082628 VERSION BG082628.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 672) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082628.1. Other_ESTs: H3078C10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3078 row: C column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..672 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3078C10-5" /db_xref="taxon:10090" /clone="H3078C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 141 c 164 g 192 t ORIGIN 1 cttggacttc cattcacagc tgcgactgaa caattgttgg gaattgggct gccgactgta 61 agtaatcaat aaattccttt actatctaga gactatccat aagttctgtg actctagaga 121 accctgacta atacacccgc catgatgaca atggactaaa gctctgaaac tgtaagccag 181 actcaattac atgattgtct tataagtatt gccttggaca tggtgagtaa caggtctgct 241 aaaaattatg tctctccaac cacgcctagt tgcttctgga tggcttatga cttactgatt 301 gtctctgggg tttaccattg actcagggtg taattaagcc agaaggtact tctctctgag 361 tatctgactt gaaagatgag aggagtccca gagggtgaga ggagactgct ttcagccatc 421 tgagagcctg atgtgacaag gatgtggatg ctcaggttgc agcagcctgt gagagcccct 481 tcagcatcct ggggtaccac acacagattt atctgtcagt gggagtgttt gtgtccccaa 541 atctgtatgt gcacaccggc atctgtgtgt atgtgccctg tcaaatgagt gaaggaattg 601 caggcatgtg ataccatggc tagaggatct gtgtcgttat cttattgact tcaaattcag 661 catcagggta ag //