LOCUS BG082589 603 bp mRNA linear EST 12-MAY-2010 DEFINITION H3077G12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3077G12 5', mRNA sequence. ACCESSION BG082589 VERSION BG082589.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 603) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082589.1. Other_ESTs: H3077G12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3077 row: G column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..603 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3077G12-5" /db_xref="taxon:10090" /clone="H3077G12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 150 a 146 c 122 g 185 t ORIGIN 1 gcattcggaa acgcgctcct tcagagactg gaagctttga aacgagatgg acagtcatga 61 ctcatgactc atcttcctcc taatggggcc gctgctggta tagaatctaa cataaggctt 121 gacacccttg cgtacagtca ctgtagaaag cgcatcattc attggctttg tgtgttcgcc 181 attccttcac acgcaggctt ttgactccca gcgtaattga gttgtcttag tttcagcttc 241 atggaagggg atttaatttt gagaccattg ctctgacact cccattctga gtttcacacc 301 cagcactctg catttcaatc cctagtcagc tcttcattct cctgttacag ggcttcaatg 361 ctgccagcac tcttttacat agaaaattct agatttgcac agtgatacag aaattagaat 421 cacctaactt cagacctgga ttcagcctgc taaatcaggg gtttactact agcttggagt 481 aactttgtag tggttatttt gtacctggcc ttattggaaa caaactgtca actagtttcc 541 cctgcacaaa ttctgaaatg tactgctctg cttaatctag tcacatgacc aagtgggctg 601 gtg //