LOCUS BG082473 574 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076E12 5', mRNA sequence. ACCESSION BG082473 VERSION BG082473.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 574) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082473.1. Other_ESTs: H3076E12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: E column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..574 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076E12-5" /db_xref="taxon:10090" /clone="H3076E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 104 a 196 c 142 g 132 t ORIGIN 1 cctgacacaa aagacgtgcc ttaaggaacc tgcctaggtg tccctctttc ctgccgcagc 61 tcacagctct atccccccac caccaccacc ccagagggca gcaagccagc tcctcccctg 121 cggaactgtt aacttattct gctctctggc tttgcacagc ctgcttgggc aggcgcaggt 181 gggtagcacc ttgggacccc caccgtcagc agcagtaccc cactgctccc ctttcttggt 241 attaagtgca attaaagctg tgggtgttgc accttctcca gagctgggtt ctcttggccc 301 cgcagcccag agaggggcat attgtgaagg gccccggcct ccagcttcca aagaggagca 361 tctggctggc tgcagggcca ggtgctccag acccgaggcc tcgccctgcg ccttccaccc 421 catcagcttg tgaccgaaac ctgctcccag agatgcttct cctctgtgca gtgcccctga 481 cccccgtaac tattgtgatt agtgagtgcc gcttgagcgg agtcagtaac ttgttgggtt 541 tttttccaac catcatggcc ttatccgtcc ccga //