LOCUS BG082394 590 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075G01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075G01 5', mRNA sequence. ACCESSION BG082394 VERSION BG082394.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 590) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082394.1. Other_ESTs: H3075G01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3075 row: G column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..590 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075G01-5" /db_xref="taxon:10090" /clone="H3075G01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 140 a 132 c 156 g 162 t ORIGIN 1 tcaggttgtc ttgaagcagg ccattaccca tgagactact gctggctcgc tgttgcctgt 61 ggtcatcatt gcagtgggtg ccttcctctt cctggtggcc tttgtgggct gctgtggggc 121 ctgcaaggag aactactgtc tcatgattac atttgccatc ttcctgtctc ttatcatgct 181 tgtggaggtg gctgtggcca ttgctggcta tgtgtttaga gaccaggtga agtcagagtt 241 taataaaagc ttccagcagc agatgcagaa ttaccttaaa gacaacaaaa cagccactat 301 tttggacaaa ttgcagaaag aaaataactg ctgtggagct tctaactaca cagactggga 361 aaacatcccc ggcatggcca aggacagagt ccccgattct tgctgcatca acataactgt 421 gggctgtggg aatgatttca aggaatccac tatccatacc cagggctgcg tggagactat 481 agcaatatgg ctaaggaaga acatactgct ggtggctgca gcggccctgg gcattgcttt 541 tgtggaggtc ttgggaatta tcttctcctg ctgtctggtg aagagtattc //