LOCUS BG082045 568 bp mRNA linear EST 12-MAY-2010 DEFINITION H3071H03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3071H03 5', mRNA sequence. ACCESSION BG082045 VERSION BG082045.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 568) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082045.1. Other_ESTs: H3071H03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3071 row: H column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..568 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3071H03-5" /db_xref="taxon:10090" /clone="H3071H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 153 a 149 c 156 g 110 t ORIGIN 1 ggagatctgg tgccctcttc tagcctctga ggacggacac cacacgtgca ctcagtccac 61 agacacacat gtaggcaaac actcatgaaa tagtaacaaa tcttagggga aagctggcac 121 atgcagcggg agggttttag gaattttatt cccagctggt tccgagggga gccttgtgcg 181 ccactgtgac atcatcgggg acctttctct aaaagtgaaa aagagagcaa gcacgtggcc 241 tggcctccta cgcctggaca tagacccgaa ggactcatgg cctactgcag agatgctgga 301 catagacccg aaggactcat ggcctactgc agagatgctg gacatagacc cgaaggactc 361 atggcctact gcagagatgc tggacataga cccgaaggac tcatggccta ctgcagagat 421 gctggacaca gacccgaagg actcatggcc tactgcagag atgctggaca tagacccgaa 481 ggactcatgg cctactgcag agatgctgga catagacccg aaggactcat ggcctactgc 541 agagatgctg gcttgcccat tgtttact //