LOCUS BG082038 584 bp mRNA linear EST 12-MAY-2010 DEFINITION H3071G08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3071G08 5', mRNA sequence. ACCESSION BG082038 VERSION BG082038.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 584) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082038.1. Other_ESTs: H3071G08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3071 row: G column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..584 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3071G08-5" /db_xref="taxon:10090" /clone="H3071G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 128 a 181 c 148 g 127 t ORIGIN 1 ctgagtggct ggtgctgact gtgctgcgtg acacgaagcc agcctgcgcc tacgagccca 61 tggattttgt gatggcgctc atctacgaca tggtgctgct ggccatcacc ctggcccagt 121 ccctcttcac gctgtgtggc aagttcaaac ggtggaaggt gaacggagcc ttcatcctcg 181 tcactacctt cctctctgcg ctcatctggg tggtctggat gaccatgtac ctcttcggca 241 actcattaat taagcaggga gatgcctgga gcgaccccac cttggccatt acactggcag 301 ccagcggatg ggtctttgtc atcttccatg ccattccgga gatccactac acccttctcc 361 cacccctgca ggagaaccca cccaactact tcgacacttc acagcccagg atgcgggaga 421 cagcatttga tgaggaaatg cacctgcctc gggcctacat ggagaacaag gccttctcaa 481 tggatgaaca taacgcaggt aaagcaacca ctgtctggtc tgaggctgtc cctgaaaagc 541 ctctccagca ggggcagatg ctcccaggcc cagcaatcac gaat //