LOCUS BG081985 549 bp mRNA linear EST 12-MAY-2010 DEFINITION H3071B10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3071B10 5', mRNA sequence. ACCESSION BG081985 VERSION BG081985.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 549) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081985.1. Other_ESTs: H3071B10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3071 row: B column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..549 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3071B10-5" /db_xref="taxon:10090" /clone="H3071B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 145 c 95 g 155 t ORIGIN 1 ggcattccac tttctagaaa agacaaccca gaatttggtc aggcctttga caatggaagg 61 cttttagaga atggcattgt taaaactact cccatagggc tgtcctgatc agggcttggt 121 tcttcctgaa atacttgttt gcactgagct acccaccact tggggtgctt tgcctggaac 181 tttaactctt actttgagca cctacataga agctgtttgg cttaaactct aacatgcact 241 gacccttgga atcagtcccc tgctccattt ggtcacccat gacctcatat taccatatct 301 accatcaagt catgtatacc agcataaggc aaatgtatac atactctatc tgtagcccag 361 ttattacagg gagggttcta gttgtcctct acaaatgctt ccaccccacc ccagcccccc 421 cccacacaca cacaaagaat tcactcaggt gcactgttgt acacacacag tgacgaggca 481 gcactcaaaa ttgaaaaagt ttaattatgg ataaacccca taattttgac cctttttcat 541 ttttaagaa //