LOCUS BG081752 655 bp mRNA linear EST 26-JAN-2001
DEFINITION H3068F10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3068F10 5', mRNA sequence.
ACCESSION BG081752
VERSION BG081752.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 655)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3068F10-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3068 row: F column: 10
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..655
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3068F10-5"
/db_xref="taxon:10090"
/clone="H3068F10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 164 a 162 c 189 g 140 t
ORIGIN
1 actcccgacc cggggaggta gtgacgaaaa ataacaatac aggactcttt cgaggccctg
61 taattggaat gagtccactt taaatccttt aacgaggatc cattggaggg catgtctggt
121 gccagcagcc gcggtatttc cagctccaat agcgtatatt aaagttgctg cagttaaaaa
181 gctcgtagtt ggatcttggg agcgggcggg cggtccgccg cgaggcgagt taccgcccgt
241 ccccgcccct tgcctctcgg cgccccctcg atgctcttag ctgagtgtcc cgcggggccc
301 gaagcgttta ctttgaaaaa attagagtgt tcaaagcagg cccgagccgc ctggataccg
361 cagctaggaa taatggaata ggaccgcggt tctattttgt tggttttcgg aactgaggcc
421 atgattaaga gggacggccg ggggcattcg tattgcgccg ctagaggtga aattcttgga
481 ccggcgcaag acggaccaga gcgaaagcat ttgccaagaa tgttttcatt aatcaagaac
541 gaaagtcgga ggttcgaaga cgatcagata ccgtcgtagt tccgaccata aacgatgccg
601 actggcgatg cggcggcgtt attcaaaaaa aaaaaaaaag ggcggccgcg ccccc
//