LOCUS BG081699 609 bp mRNA linear EST 12-MAY-2010
DEFINITION H3068B03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3068B03 5', mRNA sequence.
ACCESSION BG081699
VERSION BG081699.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 609)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG081699.1.
Other_ESTs: H3068B03-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3068 row: B column: 03
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..609
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3068B03-5"
/db_xref="taxon:10090"
/clone="H3068B03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 173 a 134 c 154 g 148 t
ORIGIN
1 gaacatccct ccatggcctc tacatcagct cctgctttct gacctgcttg agtttcagtc
61 ctgacttcct tggtgatgaa cagcagtatg gaagttagct gatgtcaatt tgataggaac
121 tcaaagaaga caagcactgt gcttcttcta agccctgaac agaagtgggt tttcatcgta
181 ccttgtccta acttgaccca catccatgat gatttttgtt gatttcgtgg gattgagaag
241 cactgtagga aagaaacaca gctctgcaag ggaagtcatg gagcacagga ggcccagagt
301 cttcaaagcc aagagaagcc cgtccggtaa agcggtgaag cggtgaaggc gaggcctgat
361 ggagcatgcc tttaatccat cgtggggaag gcagaagcag gtgaagtcct tccttggtaa
421 gggacaagcc aaggacagcc agcctgtgct cagaggtagc tggaactaaa atgctaccct
481 tcaagggact acaaatgaaa catggcatcc ctaaatttgc ctctttgcat gaggattagt
541 tttattcagg aaaacagaaa cagcagaagc tcagggaaca agaatgttta ctgctgctct
601 ttcttaaag
//