LOCUS BG081594 630 bp mRNA linear EST 12-MAY-2010 DEFINITION H3066H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066H10 5', mRNA sequence. ACCESSION BG081594 VERSION BG081594.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 630) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081594.1. Other_ESTs: H3066H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..630 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066H10-5" /db_xref="taxon:10090" /clone="H3066H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 205 a 85 c 96 g 244 t ORIGIN 1 gatagaactg ctttaaaaac aattcagtgg tcatattttt gagtgatttt tcagaaaatg 61 tagaattcat tttgtagtaa agtagtttat tttttttaat ttcaagtgtt gtaatttaaa 121 acttaagttg tgtttttaaa cagcaacaaa actgtattgt atttttgctg taattaactg 181 tataatgtaa acctaattat tttatcatgg tttaaatttt gcatatttgc tttatcttat 241 gctgctgatt tttttactga atttgtaaaa tttttgttta tcaaagcaac tattatgtgg 301 tgacttgcat atatcatgaa ttatttaaga tgtttataag ttttttatta gaatttattt 361 cagatgtttg ttcatgatgc tatccttcag ggttatgtgc ttatcaatga aataacccca 421 gaggaataag ggaaaataac ctgtagccag ttatattcag gaataactac tgtaaatgat 481 gaacgcatca agaaacctcc aatatttgct acttgccaat cccaacttag ttaaaacaat 541 tggtaggcaa ccttacctaa attttagcaa acaccttatc taatggcaga ataactcaga 601 gcatgtcttt aaagatgctg ggcacatcct //