LOCUS BG081547 526 bp mRNA linear EST 12-MAY-2010 DEFINITION H3066D10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066D10 5', mRNA sequence. ACCESSION BG081547 VERSION BG081547.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 526) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081547.1. Other_ESTs: H3066D10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: D column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..526 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066D10-5" /db_xref="taxon:10090" /clone="H3066D10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 108 a 136 c 135 g 147 t ORIGIN 1 gtccagtttg aagatggaca gaagatcgtg gtgcaaggag agccggggga tgagttcttc 61 atcattttag agggcacagc tgctgtgctg cagcgtcggt cagaaaacga agaatttgtt 121 gaagtgggac gactggggcc ttctgattat tttggtgaaa ttgccctgct gatgaatcgt 181 cctcgggctg ccactgtggt tgcccggggc cctttgaagt gcgttaagtt ggaccggcct 241 cggtttgaac gcgtccttgg cccgtgctca gacatcctca agcggaacat ccagcagtac 301 aacagcttcg tgtccctgtc cgtctgaccc gcgcctcctg tgcctcctcc ccagtccatg 361 cttcactcac gcagactgct ttctctccct agccgcagcg cccagtggcc actgcttcac 421 agcttcttgt ctctttatac taaaagttgc tttattgcac catttttcat tgggagcgtt 481 attaaatgct cattcacaaa taaatagaga gttgtatgga aatttt //