LOCUS BG081460 596 bp mRNA linear EST 12-MAY-2010 DEFINITION H3065D09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3065D09 5', mRNA sequence. ACCESSION BG081460 VERSION BG081460.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 596) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081460.1. Other_ESTs: H3065D09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3065 row: D column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..596 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3065D09-5" /db_xref="taxon:10090" /clone="H3065D09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 114 c 131 g 169 t ORIGIN 1 catcgatggt ccctggggca ggaatgggtg tatgggatgg aaccattgtt tgttcggaaa 61 gcaaattatt acacaaaaca agctttccag gtgaagatgg cttcagaagg caagtcagta 121 ctggagtcaa ggtgcattcc tgcaactgcc gctgtaagcg tactgttaat ctggatccac 181 acgatcttga aaaactcatt tgcatgattg aaatgaccga agatccttct gttcatgaaa 241 tagccactaa tgctctatat aacagtgctg attatcctta tccccaggaa attgatcgga 301 atataggtgg aatttcagtt atccaaagct tattgagcaa tccctaccca aatgtccggc 361 agaaggcttt aaatgcgctg aataacatct cagttgctgc tgaaaaccat agaacggtta 421 agacatattt aagtcaagta tgtgaagata ctgtcactta tcccttgaat tcaaatgtgc 481 aagtggctgg actaagattg ataaagcacc tgactattac tagtgaatat cagcatatgg 541 ttaccaatta tatttcagaa tttctccgtt tgttggctct gggaagtgga gaaacg //