LOCUS BG081334 679 bp mRNA linear EST 12-MAY-2010 DEFINITION H3063H11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3063H11 5', mRNA sequence. ACCESSION BG081334 VERSION BG081334.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 679) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081334.1. Other_ESTs: H3063H11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3063 row: H column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..679 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3063H11-5" /db_xref="taxon:10090" /clone="H3063H11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 150 a 199 c 171 g 159 t ORIGIN 1 cctgtgtagt ggttaaatag tgcctcagaa gtgagggtct taagaacggt catgctgttg 61 gccaggcaaa acactcttgt atgcctggtg cctagtgacc cgagagtcag cattgctgct 121 ctggcttcta tcataagcca gttagcaggg caggcagatt tcacgcagag tcggtaccac 181 cgtgatgcca gcggggttcc ctctgctttc tcacaccaca gattcccgtc acctctacgt 241 ccaacctcag ccacagctat ggacaacaac tgctaggccc agcttagacc agaggctgct 301 tctgtgtgag actgagcctg cctgcagcct gtgctggctc tgcctgggac ctacagcatc 361 ttcagtgaac aacaggatgg gaatgtactc tatccttggg aatcatccct aagccaggct 421 tcacaacacc cgcaaggcta agcatacacc agctttgcta cccgtgacat cgggtcgtat 481 tacctaggaa actccaagtg ctttctcggt gtgttacagg gaccaggaca gtgccttgta 541 ccagtttcca ggtctgtgct cgcctcctga ccatgaccac atctgccccc agagaaaaga 601 ggccctggga gctgttctct ttgctttctt gcctgctcca agagaaccac gagggggcgc 661 tgtgcacaca ctagctagc //