LOCUS BG081161 589 bp mRNA linear EST 12-MAY-2010 DEFINITION H3061G09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3061G09 5', mRNA sequence. ACCESSION BG081161 VERSION BG081161.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 589) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081161.1. Other_ESTs: H3061G09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3061 row: G column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..589 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3061G09-5" /db_xref="taxon:10090" /clone="H3061G09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 153 a 127 c 150 g 159 t ORIGIN 1 tgagcataca tatatagctc ctaaagccag aattgccttt ctacggagca accagctgtc 61 tggaattgct ttgttctaag gcaagtaggc gcctacagga gacaggaatg agtgacctcc 121 gcaggaaggg atggtggaat gtgcctgact acttctacag cccactggtg tttgacatgg 181 aagaggacca ggaagactac atatttggcc ctgatgatga ataccttcac accctggagg 241 tccacagcaa caccctcatc cagctggaga gatggttctc acccacaggc caaacccgag 301 tcactgtggt ggggccactt aaggcaaggc tgtgggtgat ggacatgatc agaaaagtgg 361 ggagtaagaa cactctcgat aaaattaaag gcaagttgat gctactccat atccggagtc 421 atcctctgac ggatcaagac ctgcagattc atctgatatc agggagcagt tgttggttcc 481 ctgattgagt gattggactt ctgtgttagt tcttagttac ttgacgtttc ctttgacagt 541 tgtttggggt ttctgttaga atttcttgta ttcataattt cacagatct //