LOCUS BG081015 679 bp mRNA linear EST 12-MAY-2010 DEFINITION H3060B05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3060B05 5', mRNA sequence. ACCESSION BG081015 VERSION BG081015.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 679) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081015.1. Other_ESTs: H3060B05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3060 row: B column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..679 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3060B05-5" /db_xref="taxon:10090" /clone="H3060B05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 177 c 193 g 143 t ORIGIN 1 ggctcagaag aggaaactga ggcacagaag ggagatggta gttctcaacc acccagacaa 61 gaaaacaaaa ggaaggccct gtaccctgac caccagaagg gatctggagt gcagaaagtc 121 gtctgctgat tgccccaatc ttgggtccag aaacactgct agacctgccc tcccttggtt 181 cttctggagg ccagaggtac agcagagagc catgagcagc ccagccatct tgggagacca 241 gagtgagaca ccataggagt ggggctgtcg tttcatcatg accctcctga gtcctggctc 301 tcctttcctg cccaggaggc taaccttcca ggtcaaagtt catagtgcat ctgggcagcg 361 gcaggtgtca cagagaacca agaagagtga gtgatggaag cctggttggt ctgactccca 421 ggtcacctgg aaccagaggt ccactgagcc tcccagattc cattccactt gcccacccag 481 gaagcctcac tgtactgatg ggggtggcca gagatctcca gggcccttgg atgctgagtg 541 accttggcaa gatccttgct gataaagact ctcctggtct cggttactca tgtgtaagat 601 ggggcaatgt tgactttaca agactcatag gatgagttaa gagggacggt ggcatcctca 661 ggtgttggcc cagtgcctt //