LOCUS BG080975 601 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059F08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059F08 5', mRNA sequence. ACCESSION BG080975 VERSION BG080975.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 601) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080975.1. Other_ESTs: H3059F08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: F column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..601 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059F08-5" /db_xref="taxon:10090" /clone="H3059F08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 119 a 180 c 167 g 135 t ORIGIN 1 gtgggggaaa tgagcttcac ctggctctcc cccaccccca ggtgaatcta cctttgtctc 61 tttgttaggc tgcagtcggt tgctgctggc tcggctgggc tgtttgggaa atgggagttt 121 tgctggcgtg agtcagcact gccctgggtg aagcctgagc tctgttgcaa cgttgcttac 181 caaagctccg tccccaggcg gcaccattca ctgtccctga gagcagctgc tgctggcccc 241 tgagatgatc tcatccgaac atggctccag cgacctcagc cgcagcttct tcgggcagcc 301 cactgtctgc tgtggcagtg ttttgagaca ggcaaaagca gattttatca tgtggccatc 361 ctgctgcaac tggttctgct tggatggaca gcctgaggag gcaccaccac cacagggagc 421 caggacacaa gcctattcca accctgggta cagctccttc ccttccccca caggctcgga 481 acctagctgc aaggcctgcg gggtccattt cgcaagcaca acccggaagc agacctgctt 541 ggactgtaag aaaaacttct gcatgacttg ttcgagccaa gaggggaatg ggccacgcct 601 a //