LOCUS BG080892 651 bp mRNA linear EST 12-MAY-2010 DEFINITION H3058F10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3058F10 5', mRNA sequence. ACCESSION BG080892 VERSION BG080892.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 651) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080892.1. Other_ESTs: H3058F10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3058 row: F column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..651 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3058F10-5" /db_xref="taxon:10090" /clone="H3058F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 185 c 152 g 139 t ORIGIN 1 ctaagttccc ccaagtgatc ccagtggtga aaggcctaga ggcctagaaa ccagcaccct 61 gtgacctctc acagcatggc ccatgcatgc tgcagtcgcc atgacagcca gctggaccag 121 gaagcttaaa cttagacaca gctcccacca aactgcccag ttagaaccgc aggtgacctg 181 ggcacccctg ggagcaggag aaatggctct ccacacatag ctaactcttt cccaaaccac 241 tatccataca tgattcactc ctgccttcaa agaccaagct caaagacaag tacaggagac 301 aaacatggag gtggtgagga ggaggccccc gcctctctct ggggcacagc tcaggtggta 361 agctctgtta cgctctattt aaaatcacag gtgtgtggat cgtaagttca taaaattctt 421 cttataagca ttccttctgg tggatgtttt tctgtagcaa ggtaagaggt aggggatagg 481 acaatctggc cccctaactg acagctgtca gactctcttg tgatgcccgg ccaccttgac 541 agaagaaacg ttaggcagcc agccagtcag cctatcctta tgccagcacc agccattttg 601 agcaaccctt agaagcctta caaaaggcac agcttcagcc agggctgctt c //