LOCUS BG080842 698 bp mRNA linear EST 12-MAY-2010 DEFINITION H3058B05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3058B05 5', mRNA sequence. ACCESSION BG080842 VERSION BG080842.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 698) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080842.1. Other_ESTs: H3058B05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3058 row: B column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..698 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3058B05-5" /db_xref="taxon:10090" /clone="H3058B05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 146 a 179 c 144 g 229 t ORIGIN 1 tccgtacagt taagtctgag ctctttctgg acctcacttc cttccctatg atgtttatgc 61 tgcatttcca tggcccctct tctccgaaga gttttgcggc ccggagagag ttgtgaagag 121 caagggaccc tagactggct tttcttgcta ctgctggggc tgcgattggt ggttcctgtt 181 cgttgttatt tatttccctg ttctagctcc tttggtatcc gcccctcttc gggaccatca 241 taatccacac gagtcaaact gggagtccat gtgctttttc ctcagattcg aaaattggaa 301 ggtaacaggc acttcgggat tcccgttccg ccttctggtt tagggattgc tactggtgtt 361 ggacgtcttt ggatccttat ttaataaata gaacacgtgt gctttcctta ccattacatc 421 gctccaaaca catagtcttg atttgtttgt aacggagaaa caaaaaaagt ctcactttca 481 agaactgcgc ttccatatcc tcgggccccc aataccttaa ttttacgtag tggagtgaaa 541 ctgttttatt tttaagtttc attcaactca ctgcagcatg accttctatt tgtctggttt 601 tcggagcatc ccgaaacttt ggaagagcaa cccttacttt gagctaggcc ctgcgacctc 661 ttctaccccc ttctttctct gtgccatcgg gaaccaac //