LOCUS BG080667 605 bp mRNA linear EST 12-MAY-2010 DEFINITION H3057B08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3057B08 5', mRNA sequence. ACCESSION BG080667 VERSION BG080667.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 605) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080667.1. Other_ESTs: H3057B08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3057 row: B column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..605 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3057B08-5" /db_xref="taxon:10090" /clone="H3057B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 153 c 141 g 129 t ORIGIN 1 cgagtgggac accacaaacc actgcagact cggccaccag ccccaagctc ctctatgtag 61 atagagttgt acaggaaatc ctggagacag agaggaccta cgtgcaagat ctaaaaagca 121 tcgtggaggg ttacctggaa tgcatacggg accaaaccaa acttcccctg gtgaccgaag 181 acagggctgc tctttttgga aatatacagg atatctacca cttcaatagc gaacttcttc 241 aagatctgga aaactgtgaa aacgatcctg tggccatagc agaatgtttt gtgtccaaga 301 gtgaagagtt ccacatttat acccagtatt gcaccaatta cccaaggtct gtggccgtgc 361 taactgagtg catgaggaac aagatactca ccaagttctt ccgggagcgc caggaaactc 421 tgagacactc actaccccta gggtcctacc tcctaaaacc agtccagcgg attctcaaat 481 atcatctcct tcttcatgaa atagagaacc accttgataa ggccagggaa ggctacgatg 541 ttgtgctgga tgccatagac acgatgcagc gagtggcctg gcacatcaat gacatgaaac 601 ggaag //