LOCUS BG080643 582 bp mRNA linear EST 26-JAN-2001 DEFINITION H3056H08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3056H08 5', mRNA sequence. ACCESSION BG080643 VERSION BG080643.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 582) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3056H08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3056 row: H column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..582 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3056H08-5" /db_xref="taxon:10090" /clone="H3056H08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 116 c 110 g 172 t ORIGIN 1 aataccttct ggtttttgtg aacgttctca agatagataa aagcttttcc ttgtaaaact 61 gagactgctc agatagtcat caagaagatt attaaaaatt ttccaaggtt cggagtgcca 121 aaagcaatag tgtcagataa tggtcctgcc tttgttgccc aggtaagtca gggtgtggcc 181 aagtatttag aggtcaaata aaaattttat tatgtgtaca gacctcagaa ctcaggacag 241 atagaaataa taaataaaac tctaaacaga ccttgacaaa attaatccta gagactggca 301 cagacttact tggtactcct tccccttgcc ctatttagaa ctgaaaatac tccctcttaa 361 ttcggtttta ctctttttaa gatcctttat ggggctcctg tgcccatcac tgtcttaaat 421 gatgtgttta aacctatgtt gttataataa tgatctatat gttaagttaa aaggcttgca 481 ggtggtgcag aaaaaagtct ggtcacaact ggctacagtg aacgagccag gtaccccaag 541 gacaactcac cagttccagc cagagatctg atctacgtac ac //