LOCUS BG080496 627 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054D02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054D02 5', mRNA sequence. ACCESSION BG080496 VERSION BG080496.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 627) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080496.1. Other_ESTs: H3054D02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: D column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..627 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054D02-5" /db_xref="taxon:10090" /clone="H3054D02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 155 c 177 g 148 t ORIGIN 1 gttcgttggt ggagtatctg cccgttctgc tcgccgattg cggctcttgt tcgagcgctt 61 ccagcgtcgc ctcggtctca gcaagggccc cagactcggc tttgggtgaa aaaaaatggc 121 ccgaaccaag cagaccgcta ggaagtccac cggtgggaaa gccccccgca aacagctggc 181 caccaaggcg gctcggaaaa gcgcgccctc taccggcggg gtgaagaagc ctcaccgcta 241 caggccaggg accgtggctc tgagagagat ccgtcgttac cagaaatcga ctgagctgct 301 catccggaag ctgccattcc agagattggt gagggagatc gcccaggatt tcaaaaccga 361 cttgaggttt caaagtgcag ccatcggtgc ccttcaggag gctagcgaag catacctggt 421 ggggttgttt gaagatacca atctgtgtgc catccacgcc aagagagtca ccatcatgcc 481 caaagacatc cagttggctc gccggatacg gggggagaga gcttaagttg aagcggtttt 541 tatggcattt tgtagtaaat tctgtaaaat actttggttt aatttgtgac tttttttgta 601 agaaattgtt tataatatgt tgcattt //