LOCUS BG080440 688 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053F09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053F09 5', mRNA sequence. ACCESSION BG080440 VERSION BG080440.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 688) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080440.1. Other_ESTs: H3053F09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: F column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..688 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053F09-5" /db_xref="taxon:10090" /clone="H3053F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 251 a 130 c 152 g 155 t ORIGIN 1 tcatgtacac tggtctttgt tgtgtaaacc ttgctcccca atttaaattg ataaaaggcc 61 tgtgattggg cagagagaga aatgtaggac ctgagaacca actgaggggt cttgagagag 121 accacagggg agaaggagag agaagatgga gagggaggag gccacgtgat caggagaagg 181 gcatcctgag gacaggctga tggagcaaaa gcagcccaga tgaggctcac acagaaggta 241 gcaaggggct atgatctgga tatgggtgag aacagtgtag aacctgccca atctaggctc 301 acagtgtgta aataaaatgc tacgtttatt tgggctagct agaataaata tttcattaac 361 aggatttgat tcccacagct gcgtgtaact ccagtgccag gggacctcat gccctttgtg 421 cctctgtggg cacgcacata aagtaccgac atacatgagg acaagcaccc acacaggaaa 481 taaataatag aatagatata gacaataata tacagataga tgtgtagaat atatctatag 541 aactacactt tatataatat ataccatata taaaattgat agaaaatagc atgcatataa 601 tatatataca tatatacata cacatatata tacatacaca cacacacaca cacacacaca 661 cacacacaca cacatatata tatgaatc //