LOCUS BG080360 642 bp mRNA linear EST 12-MAY-2010 DEFINITION H3052G02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3052G02 5', mRNA sequence. ACCESSION BG080360 VERSION BG080360.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 642) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080360.1. Other_ESTs: H3052G02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3052 row: G column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..642 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3052G02-5" /db_xref="taxon:10090" /clone="H3052G02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 183 a 147 c 163 g 149 t ORIGIN 1 cattgatcct gtttcttagg acactgttcc tttccatttc tcatcagctc atccaggatg 61 ctgagcaccc tgtcccacaa ggacagcagg gatagaggag agcctttgaa gtcagaagag 121 ctagctaaca taccagctcc agaggtggct agtgcttaag gggacacact gtatacttgg 181 gaaacacaaa gagaagccaa ctgtttccat ctgtggcttc ttgagaagtg tcagtgagac 241 aggggtaggg gcactcgtga agtaaaaacg gaaatgataa taaccatatc tctaaggcta 301 ccagggagga aagccatctc ataaacatgg aaatggaaat caatggatgg ttgagttctt 361 agaagaaaga gccatgggac tgtaatcact gaagggcaca gaagtgctta gtcacccagc 421 atcatgggaa aacattgctg aattttgatc caccttatta atcaccaaac cttgagggtg 481 ggctttctgc cttggtttct cacattctct cagtctaacg ctgagaacgt aagctgtagg 541 caaacagctg caagctgaaa cccatccttg cctcctttct gcagctatgc caggcctggg 601 cacaaggcat gccaggggca gggcagtgga agagccctgg ct //