LOCUS BG080309 724 bp mRNA linear EST 26-JAN-2001 DEFINITION H3052B04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3052B04 5', mRNA sequence. ACCESSION BG080309 VERSION BG080309.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 724) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3052B04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3052 row: B column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..724 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3052B04-5" /db_xref="taxon:10090" /clone="H3052B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 221 a 143 c 148 g 212 t ORIGIN 1 ctagacaaag caacccagta gggagaaagg atctcaaaga caggcagagt cagagaccat 61 gcctgatccc actgttagga gttccccgtg gagaatggcc tatacaattg aaacatatat 121 gtgggggggc ctgggccagt cccaagactg ctccttgttt ggcagttcag acttgtaagc 181 ccagataaac tcgagttagt tgattctgtg gattttcttc cagtgttctt gcctcctctg 241 gctcctacaa tctttcctcc tcctctttca agggattccc taagctcacc taatgttttg 301 gctgtttggt ctttgcatct gttcccatca gttgttgggt gaaacctttc tgaggaggac 361 taggttaggt gccgatttat aagtatatca gaataccatt aggaattatt tcattgactt 421 cagttttttt ctttgtgcct ttggttctat agaggcacct ttaatcataa taaagagaaa 481 gtagagacaa cctaaatgtc tgtcagctaa agaatggata aagaaaatgt ggtacactca 541 tatactggag tattgcttag atattacaaa caataacatc ataaaatttg ctggcaaatg 601 gataaaagtt catcctgaca aaggtaaacc aaaccccaaa agattaatat ggtatgcaat 661 cacttataag tggatattag tcataaagta aaggataacc atgctacaat acacagactc 721 aaag //