LOCUS BG080274 654 bp mRNA linear EST 12-MAY-2010 DEFINITION H3051G04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3051G04 5', mRNA sequence. ACCESSION BG080274 VERSION BG080274.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 654) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080274.1. Other_ESTs: H3051G04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3051 row: G column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..654 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3051G04-5" /db_xref="taxon:10090" /clone="H3051G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 184 c 200 g 132 t ORIGIN 1 gtgggaccaa gggggcatca cccacctgga ccgggctgag caggcccgta tcctgcagag 61 ccgagaggtc ccccggcatc tctgttgccg gaacccagag tggctattcc gaatctacca 121 ggacaccagg gtggacatcc catcccttat gcaatccatt cggcgtgtgg tgaagggccg 181 gccggggcca cggaggcaga ggtgggcaat cagcctgtac ccgggcaagg tgcgggaggc 241 tcgatgtcag gcgtcagtgc agggtgccac cgaggcccgc ctgtctgtgt cctggcagat 301 cccgtggaac ctcaagtacc tgaaagtgag ggaggtgaaa tacgaggtgt ggctgcagga 361 gcaaggggag aacacgtatg tgccttacat gctgaccctg cagaaccaca ccttcacaga 421 gaacatcaaa ccttttacca cctacctggt gtgggtccgc tgtatattta acaggagcct 481 cctgggacct tttgcagatg tgctggtgtg cagcacgtag ccagcagacc atggccaggc 541 ctctggaggg cacctctcgc agctcagctt tcccaggtcc acagttctgc tagcagctgt 601 ggtgataaat tatctattta ttgagtaggc ttgcactaag cctgcttccc gagt //