LOCUS BG080259 557 bp mRNA linear EST 12-MAY-2010 DEFINITION H3051E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3051E12 5', mRNA sequence. ACCESSION BG080259 VERSION BG080259.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 557) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080259.1. Other_ESTs: H3051E12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3051 row: E column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..557 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3051E12-5" /db_xref="taxon:10090" /clone="H3051E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 125 a 176 c 135 g 121 t ORIGIN 1 cctccccttt aacggcggca actccacgct ccgctgaccc caaagccgct gccgctgcta 61 acagcggctc ctgctgcctc agccgccggc ctcgggcagc tgcatccata ccccgggctg 121 ggtccccgcc ccgccatggt gtcctggatc atctctcgcc tggtggtgct catctttggc 181 accctgtacc cagcctattc ttcctacaag gccgtgaaga ccaaaaacgt gaaggaatac 241 gtaaaatgga tgatgtattg gatagtcttc gccttcttca ccacagctga gacacttaca 301 gatataatac tgtcctggtt ccccttctac tttgagctca agattgcctt tgtgatatgg 361 ctgttgtccc cttacaccaa gggctccagt gtcctctacc gcaagttcgt gcacccaaca 421 ctgtccaaca aggaaaagga gatcgacgaa tacatcacac aagctcgaga caagagctat 481 gagacgatga tgagggtggg caagaggggc ctgaacctgg ctgccaatgc tgcagtcaca 541 gctgctgcca agggcca //