LOCUS BG080229 693 bp mRNA linear EST 12-MAY-2010 DEFINITION H3051C03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3051C03 5', mRNA sequence. ACCESSION BG080229 VERSION BG080229.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 693) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080229.1. Other_ESTs: H3051C03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3051 row: C column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..693 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3051C03-5" /db_xref="taxon:10090" /clone="H3051C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 172 c 204 g 154 t ORIGIN 1 caagtgctgc caccttattt actccagctt ggaccgagct aatcagagga aaagtgagct 61 gtgctgtggc tcccgcggct gactggctga gtgactggct gagtatagac aactgtcatc 121 atgccccact catacccagc tctttctgct gagcagaaga aggagttgtc ggatattgct 181 ctacggatcg tgacccccgg caagggcatt ctggctgcag atgagtccgt aggcagcatg 241 gccaaaaggc tgagccaaat tggggtggag aacactgagg agaatcgccg gctgtaccgc 301 caggtcctat tcagtgctga tgaccgtgtg aaaaagtgca ttgggggggt catcttcttc 361 catgagacac tctaccagaa agatgacaat ggtgtcccct tcgtccgcac catccaggat 421 aagggcattc tcgtaggcat caaggttgac aagggtgtag tgcctctagc tgggaccgac 481 ggggaaacca ccactcaagg gctggatggg ctcttggaac gctgtgctca gtataagaag 541 gacggtgctg attttgccaa atggcgctgt gtactaaaaa tcagtgatcg cacgccgtcg 601 gcactggcca tattggagaa tgccaacgtg ctggcccgct atgccagcat ctgccagcag 661 aatgggatcg tgcctattgt ggagcctgag att //