LOCUS BG080014 679 bp mRNA linear EST 12-MAY-2010 DEFINITION H3047G08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3047G08 5', mRNA sequence. ACCESSION BG080014 VERSION BG080014.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 679) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080014.1. Other_ESTs: H3047G08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3047 row: G column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..679 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3047G08-5" /db_xref="taxon:10090" /clone="H3047G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 189 a 144 c 174 g 172 t ORIGIN 1 actatgacat catttggcct tcaggttttg tgtgtgacaa ctgtttgaag aaaactggca 61 gacctcggaa agaaaacaaa ttcagtgcta agaggctgca gaccacacga ttgggaaacc 121 acttagaaga cagagtgaat aagtttttgc ggtgccagaa tcaccctgaa gctggggagg 181 tttttgtcag agtggtggcc agctcagaca agactgtgga ggtcaagccg ggaatgaagt 241 caaggtttgt ggattctgga gagatgtcgg aatctttccc atatcgtacc aaagcactct 301 ttgcttttga ggagatcgat ggagtcgatg tgtgcttttt tgggatgcat gtgcaagaat 361 acggctctga ttgcccccca ccaaatacaa ggcgtgtata catatcttat ctggacagta 421 ttcatttctt ccggccccgc tgcctccgga cagctgttta ccatgagatc ctcatcggat 481 atctcgagta tgtgaagaaa ttggggtatg tgacaggaca tatttgggcc tgtcccccaa 541 gtgaaggaga tgactatatc tttcattgcc acccccctga ccagaaaatc cccaaaccaa 601 aacgactaca ggagtggtac aagaagatgc tggacaaggc gtttgcagag aggatcatta 661 acgactataa ggacatctt //