LOCUS BG079999 716 bp mRNA linear EST 12-MAY-2010 DEFINITION H3047E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3047E02 5', mRNA sequence. ACCESSION BG079999 VERSION BG079999.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 716) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079999.1. Other_ESTs: H3047E02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3047 row: E column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..716 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3047E02-5" /db_xref="taxon:10090" /clone="H3047E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 189 a 163 c 211 g 153 t ORIGIN 1 gtgaatgctg cagagatcct ttttagaaag ggaaaatagt gcagacaatt tttctgacat 61 actaactaaa ggatgacagt ccccggggca gcacggtgtc cctgcagccg agccctgtga 121 ctgtgcatat tgaccgtctc gtggtggaga ggagtgatga tggctctttc cacatcagag 181 attctcacct gtttaacact gggactgatt tcaaagacgg tgccagcagt gattcagtag 241 tgcggacccg ggggatgtgt gacgtgagga tgcacagcag tgtcacccaa gccacccaga 301 cgagcccaga ggttcctttg ccttcacagt cagccaactt tctcgacatt acaagggaac 361 agctcatgga ggaaaacgaa tgtctcaggc agagactagc tcaagctaaa atggagctcg 421 cggaggctca ctcggccaga gatgagcttc tccatcaaat gaagaggatg ggcctgtagc 481 tgccgtggcc gtgagaaccg gggctagagc gctggagaag gacggctagg tttaacagaa 541 ggatgtaacc agttagtggg aagttttcct ttacatgaaa taaagcgagt ggtgaagtgg 601 cggctgttcc aaaccctgtg gaggaaacct tgggcgcaag cggtacagtc atctgggtgg 661 ccccgtgaag gctgcgcaca ctgtaacaca gttacatgcc taatgagaaa tgtcag //