LOCUS BG079968 612 bp mRNA linear EST 12-MAY-2010 DEFINITION H3047B02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3047B02 5', mRNA sequence. ACCESSION BG079968 VERSION BG079968.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 612) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079968.1. Other_ESTs: H3047B02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3047 row: B column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..612 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3047B02-5" /db_xref="taxon:10090" /clone="H3047B02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 112 c 138 g 215 t ORIGIN 1 tgaaaatgtt tgagagtgtg gagaaagatg ttaggctctt tgtgatttgt ctctggagtg 61 aagtgaatga ataactttac tttgtgtaaa ggaatttttt tttgtaatac ttatgtgata 121 acatcttaca ttaagtaata aaacctcaca agagacagct atatcagggt cctttcagaa 181 aaatcttgct ggcgtatgca atggtgtcag catttggagg ctgattatgg gatggatatc 241 cgggtgtggc agtctctaga tggtccatcc ttttgtctca gctccaaact ttgtctctgt 301 aactccttcc atgggtgttt tgtttccaat tctaagaagg agcatccact tatgtgtttg 361 ctaggcccca gcctagtctc acaagagaca gctatatcag ggtcctttca gcaaaatctt 421 gttagtgtat gcaatggtgt catcgtttgg aggctaatta tgggatggat ccctggatat 481 ggcagtctct agatggtcca tccttttgtc tcagctccaa actttgtctc tgtaactcct 541 tccatgggtg attgtttcca attctaagaa tgggcaaagt gtccactttg ttcttcttca 601 gtttcatgtg tt //