LOCUS BG079775 584 bp mRNA linear EST 12-MAY-2010 DEFINITION H3044H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3044H10 5', mRNA sequence. ACCESSION BG079775 VERSION BG079775.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 584) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079775.1. Other_ESTs: H3044H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3044 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..584 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3044H10-5" /db_xref="taxon:10090" /clone="H3044H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 134 a 161 c 118 g 171 t ORIGIN 1 atcccttgcg atgccatgaa gctccttaca aggaatgctg ctctgtggtc cagacctctc 61 cctctcccta cctccaagga cttctgtgca gaagaattgg tctttgctgt gggctttcat 121 ggggactgtc aaaaccctat cagcacactg gcaggagata ggagaatgtg tcctccactg 181 aaagctacag tcccttttgg acatcctgtc catgcaaccg tctctgcctg tcccagcgat 241 cacataattc ctattctcgt cccgctgcct cctgctgtac ccaggcttgg aacatggtct 301 gggcgatttc atttctcctg cctgcatctt cccaggtcat ctgtttcact aatgagatct 361 cactgagcat gctcgaactt ctccctctgg gacctctcaa cactcctggt atagattagc 421 tcattttctg ccatacagag ttgaaacaat ttcaagaggc atatttatgt ttatatgttt 481 ccaggaagca cctgttaatc aatgtggaaa ggtattgaga accgtaaccc ctggatcctc 541 caaatctttg agctattgat acgtgaaagc tcaatagcca tttg //