LOCUS BG079660 690 bp mRNA linear EST 26-JAN-2001
DEFINITION H3043C07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3043C07 5', mRNA sequence.
ACCESSION BG079660
VERSION BG079660.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 690)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3043C07-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3043 row: C column: 07
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..690
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3043C07-5"
/db_xref="taxon:10090"
/clone="H3043C07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 177 a 126 c 126 g 259 t
ORIGIN
1 ccttctctgt cttcctgttt gcatggcaat ttccttggcc tccttccttg gggccaatga
61 acttgccaag gtaaacctgc ccttttcaac tcttaattcg gtttgaattg gcttattcca
121 tctaaggaga aaacctattt tttgaaactg taaacaagcc ccaattaaat gttttctttt
181 gtaagagttg ccagggtcat agtgtctcct catagcaata tagtactgac taatacattc
241 acatacatgt acgtatgtat atgtccatgt atacatatat gaatgtatat gttcaaatat
301 atttaaatac cagtttgctg agtcttttat tggttngtgt gtatatgngt ttcaagactg
361 acctatttgt ttttttgttt atttgtttat tttatttttt attgatttag cttttcaaga
421 caggattttt ctgtgtaaaa agccctggct gttctagaac tcactttgta gaccaggttg
481 acccagagat atacaggttg tctctgtatt ataataatgc tgatttctga actggcagag
541 atccagttac aggtgggact ttgaaattgt taagcatctc ctgtctcagt gttttgtaaa
601 tactccctct ctcaccttct gattgggtta ttaaaaagct gaccgacaag atatggagag
661 gtggaccagt gtttaacagc actgactact
//