LOCUS BG079649 687 bp mRNA linear EST 12-MAY-2010 DEFINITION H3043A04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3043A04 5', mRNA sequence. ACCESSION BG079649 VERSION BG079649.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 687) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079649.1. Other_ESTs: H3043A04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3043 row: A column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..687 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3043A04-5" /db_xref="taxon:10090" /clone="H3043A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 173 a 186 c 145 g 183 t ORIGIN 1 ttgggattca gcctgcctcc tggtactacc cagttagatc agaaccggtt cactactata 61 gaagataagc tatgcagagt cccagccagt cccagttgaa gagaggtatt gagggaaata 121 tggctagact tgccaccagg ggcagctggc tagcaagcca ctcataggcc cctgactgct 181 ggtctctctt ggtggcagat ctttcctacc acttctttct gtgggattct tacagattct 241 ccatccaacc ctacctaacc gcctcagcag tcttaaaaat gacacacacc gagtttgcga 301 ttgcttcagg gacgagaaca cagtgactcc ctggatttgc tgctacaatt ctttactctc 361 tgttcttgtc gcagaaccta ttagtggctt tctttagccc taaggcaaaa ataaacctgc 421 catgaaagta gatcattgac cttaattgta aggtgactga ctaggaagaa ggacaaaggt 481 ggcagctgct aacggccatc gtctctactg tcatccttcc catgcctctg atctgctcac 541 agcacatttt acatgcatgt tgccttcctg acttctaggg attctggtgg gactgcaaat 601 caactgtcct gctctgtgcc cacacaacta accaagcttg gacttctgca tagcctatcc 661 catagtgaac caatccagag atgacac //