LOCUS BG079527 599 bp mRNA linear EST 12-MAY-2010 DEFINITION H3041E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3041E02 5', mRNA sequence. ACCESSION BG079527 VERSION BG079527.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 599) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079527.1. Other_ESTs: H3041E02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3041 row: E column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..599 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3041E02-5" /db_xref="taxon:10090" /clone="H3041E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 168 a 117 c 151 g 163 t ORIGIN 1 gggctactct aatctatggg tagggacata tgattttaga agggcccttc agtactctgt 61 ttatctgaca gtatagtagt agttggttca ccatcatgtt atgtgggtga tgtagtctag 121 tgctgcagat ggggatggag agatgactcg gtagctaagt catgctgatc ttgtagagga 181 cctaggttca attcccagta cccacatgga gactcacaat tgtctgtaac tccagttcca 241 gggatctgat gctttctgct ctcctcaggc atacttgtac acatacatcc atgcatgcaa 301 gcacttatat acataaaatt agtctttttt aaataaaaaa aaggatacat aaataagtga 361 tggaactctg cgtccccgtc cttgagggct cctgtgtgat agggaagcat tcgggaaggg 421 ataacatctt atctgctgtg catgctgtga agatagtcta gccgagtaaa ggccggagtg 481 acccagagaa gcctgaacgg cttttctggg cagacagcct ctgagcaaag gccagaataa 541 agtcactgtg tgcatagcta agggaaggac tttgaatcca tggaatagca gattcaaag //