LOCUS BG079142 659 bp mRNA linear EST 12-MAY-2010 DEFINITION H3036C10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3036C10 5', mRNA sequence. ACCESSION BG079142 VERSION BG079142.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 659) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079142.1. Other_ESTs: H3036C10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3036 row: C column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..659 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3036C10-5" /db_xref="taxon:10090" /clone="H3036C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 142 a 166 c 218 g 133 t ORIGIN 1 ccggcagctg gaggctgtgg ttgaaggcat gatagcccct cagcccccca tgcacctcag 61 tggccgctgt gtctgcccca gtgaccccca gttcgtggag gaaaagggga tccaggcaga 121 agacttggtg atcggggctc tggagaacgc cttccaggaa tgtgatgatg tgattgggag 181 agagctggag gcctcaggac aggtgggtgg ctgtacagcc ctggtggcgg tgttcctgca 241 agggaagctg tatgtggcca atgctgggga cagcagggcc atcttggtgc ggagacatga 301 gatacggcag ctgagttctg agttcacccc agaaacagag cggcagcgga tccagcagct 361 ggcctttacc taccctgagc ttctggctgg agagttcacc cgactggagt tcccacggcg 421 gctaaagggg gatgacttag ggcagaaggt tttgttcagg gatcatcaca tgagaggctg 481 gagctacaaa cgggtggaaa agtctgatct caagtatcca ctgattcatg gacagggtag 541 gcaggcgcgg ttactgggaa cactggctgt ctctagaggg ctgggcgacc atcagcttag 601 agtcctggac accgacatcc agctgaagcc cttcctactc tccatccctc aggtgactg //