LOCUS BG079083 649 bp mRNA linear EST 26-JAN-2001 DEFINITION H3035E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3035E12 5', mRNA sequence. ACCESSION BG079083 VERSION BG079083.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 649) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3035E12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3035 row: E column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..649 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3035E12-5" /db_xref="taxon:10090" /clone="H3035E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 288 a 115 c 133 g 112 t ORIGIN 1 ggaatcacag gatcacagag aaagctggac tctgaggaat tctgaatcaa gcagtattac 61 aggaaggaca ggctccaatc agatataccg agggcagcaa gcatgtgaga taataggatg 121 gcaggaggca aacgtaagag cagaaaccaa ggtgacttgg cttcatcaga aacaaacttt 181 cccaccatag caagtcctgg atacaccatc acaccagaaa agcaagatat ggatctaaag 241 tcatttctca taatgatgat gatggaggaa tttaagacag aaataaataa ctcctttata 301 gaaatacagg agaacatata caatcaggtg aaaaattgaa caaaaccatc cagaatctaa 361 aactagacct agaaacagtt aagaaatcac aaaaagagac cactgtggaa atagaaaacc 421 taggaaaaaa gtaggagtca ttgatgtaag cataaccaac agaatacagc tcatctgaaa 481 gctctagaac aaaaggaggc aaattcacca aagaggagca gaggcaggaa ataatcaaaa 541 tcagggctga aatcaaccaa gtagaaacaa aaagaactat acaaagaatc aaccaaanca 601 ggaagtgtgt tctttgagaa aatcaaaaaa aaaaaaaaaa gcggccgct //