LOCUS BG078622 589 bp mRNA linear EST 26-JAN-2001 DEFINITION H3029H11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3029H11 5', mRNA sequence. ACCESSION BG078622 VERSION BG078622.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 589) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3029H11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3029 row: H column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..589 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3029H11-5" /db_xref="taxon:10090" /clone="H3029H11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 90 a 163 c 208 g 128 t ORIGIN 1 cttccctatg taccgcagtc tcaagttcca cgcgattgga ttggggtagg cagctgtgtt 61 ccactcacca gaggtcttag ggtcccgtgg ggagtcccgt gtgggccctt gcgggtgttg 121 ggcaagactc tgctgtcaag gtagcccggg gctcgagtct cgagtcgagc ggaagggact 181 tgtgccccag atcaggcccg ggtagcctgc ttccctatgt accgcagtct caagttccgc 241 gcgattggat tggggcaggc accgtggtcc actcaccaga ggtcttaggg tcccgtgggg 301 agtcccgtgt gggcccttgc gggtgttggg caagactctg ctggcaaggt agcccggggc 361 tcgagtctca agtcgagcgg aagggacttg tgccccagat caggcccggg tagcctgctt 421 ccctatgtac cgcagtctca agttccgcgc gattggattg gggcaggcac tgtgatccac 481 tcaccagagg tcttagggtc ccgtggggag tcccgtgtgg acccttgcgg gtgttgggca 541 agactctgct ggcaaggtgg cccggggctc gagtctcgag tcgagcgga //