LOCUS BG078531 604 bp mRNA linear EST 12-MAY-2010 DEFINITION H3028H04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3028H04 5', mRNA sequence. ACCESSION BG078531 VERSION BG078531.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 604) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078531.1. Other_ESTs: H3028H04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3028 row: H column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..604 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3028H04-5" /db_xref="taxon:10090" /clone="H3028H04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 162 a 128 c 160 g 154 t ORIGIN 1 cagcgatgga gctgaagcaa agaagcgaaa gcgtgtggtc agcgagatct gtgagacaga 61 gaaccaggaa gaaacagtga aggagaatct agatctttct aaaaagcaaa aggcgttaaa 121 tcttcctgct aaccagaaat tgtcagcgtt tgcatttaag caggagtaaa ggaaggattg 181 tctgtcagga agtagtgaga cctggcttca gcttctcttg gattctggac gtcttttaac 241 ctttagggag tgtattctta taatcctgtg tgtttagaca cagcgtggtt ctcataaggc 301 agtgtagggg ctgggtgtgc agctcagtgg tggagggctt gcctaagcat gcgcaggccc 361 tggttccctt actgccgcaa aacaacacac aacaaagtgg tgattttcct gtgtcaggaa 421 caccacatgt aagagctgtc tgtggggatt cctctgctgt ccatgagcag aaatgccatg 481 atggcagtga ctaccaggga caccattctg tctccgtgga agccattatc ggtaaaagtc 541 actaaaagcc tcagtcttaa gacttgttca cattacagta cctcctaggg ctttcccgcc 601 tttg //