LOCUS BG078526 591 bp mRNA linear EST 12-MAY-2010 DEFINITION H3028G11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3028G11 5', mRNA sequence. ACCESSION BG078526 VERSION BG078526.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 591) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078526.1. Other_ESTs: H3028G11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3028 row: G column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..591 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3028G11-5" /db_xref="taxon:10090" /clone="H3028G11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 135 a 154 c 174 g 128 t ORIGIN 1 aagacatcct tgtccgctcg cctgctacct ctgaggaaac tcggggctct actcaaagtg 61 ctgaggctgc ctgctgtctc ccggggagat catgaaacga ggtcgccttc ccagcagcag 121 tgaggattct gacgacaatg gcagcttgtc aacgacatgg tcccagcatt ctcgatccca 181 gcatgggagg agtagtacct gctctagacc tgaagatcga aagccttctg aggtgtttag 241 gaccgacctg atcactgcca tgaagttgca tgactcctac cagctgaacc cggatgacta 301 ctacgtgctg gctgatccct ggagacagga gtgggagaaa ggggtccagg tgcctgtgag 361 cccagggacc atcccacagc ctgtggccag ggttgtgtct gaagagaagt ctctcatgtt 421 catcaggccc aagaagtaca tcgcgtcgtc cggctctgag cccccagcgt taggctacgt 481 cgatatccgg actctggcag acagtgtgtg tcggtatgac ctcaatgata tggatgctgc 541 gtggctggaa gtaaccaatg aagaatttaa ggagatgggg atgccggagc t //