LOCUS       BG078332                 584 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3026F12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3026F12 5', mRNA sequence.
ACCESSION   BG078332
VERSION     BG078332.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG078332.1.
            Other_ESTs: H3026F12-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3026  row: F  column: 12
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3026F12-5"
                     /db_xref="taxon:10090"
                     /clone="H3026F12"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          150 a          153 c          119 g          162 t
ORIGIN      
        1 cctccctgtc ctgatcttag aacagtggaa gtgaagaaca ccccacactc tctagcttta
       61 gaatactggg gttttggttt tggtttttta aattgcagtt gttttaagaa attcaacctt
      121 tcagaccagc ccttccacta aactttgatt ctttttcttt actgcttttt taaacctaaa
      181 aaaaaaaaaa aagaaaaaaa aaggaaagaa agcaagcgca ctcagtgact cccccacctt
      241 ggtctgtggg aatgcccgcc cgcactctgc cgtccatcct cctcccttct cctcatgcag
      301 tgtctgtgaa gtagttttat ctgtttctta ggatgaaaaa cggcaatcat cccaccaggt
      361 gctggaaact ggtcagcctt gaagcaggca cttctgggac cacagctcag ggatgtgtat
      421 ttaattacag ggtccccaga aagcctctct tggctgccac aagtagcatc tttcctggac
      481 ttggagtcag accctgacaa cttcctctga gtgagcagcc ccaggccact gaactgaacg
      541 caagcttgcc tatgggtctg ggcttcctca agacttttga ccgt
//