LOCUS       BG078319                 542 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3026E11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3026E11 5', mRNA sequence.
ACCESSION   BG078319
VERSION     BG078319.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG078319.1.
            Other_ESTs: H3026E11-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3026  row: E  column: 11
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3026E11-5"
                     /db_xref="taxon:10090"
                     /clone="H3026E11"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          119 a          114 c          141 g          168 t
ORIGIN      
        1 ggatgggtag gtttgttaga ccacgaagct tggatccccg tccccggttt ttcctttaga
       61 ccaagtcaca ctatataggc ctggcaagct tgggacttgc tgtatagacc gtctggcatt
      121 taactccagc ggtggtattg tctggagact atttccactt ggggattggt cacagggtga
      181 gggctgtctg tctgtctgca cactgtgggg atagtggttc ccttggcttg ttagcaggct
      241 cttccccttc agaccgggag ccagtccagt ttagtggagt gaattggtct tgtttgctac
      301 tgtaacccca acctccagga attttggcat aagaaagatg attaaactga gagaaaatga
      361 tttctatacc ctatgcattt ctgggtgcat attgagatgt ggctgtgcat tttagatccc
      421 ctctgaaagg gcctgtgcag gacagcagaa cttgtattac agatctcagg tggcttttac
      481 tcagtctcag gtgctgcaga atgaagccat ggccatattt aatgtcatat tgttactttt
      541 ta
//