LOCUS BG077942 652 bp mRNA linear EST 12-MAY-2010 DEFINITION H3020G07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3020G07 5', mRNA sequence. ACCESSION BG077942 VERSION BG077942.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 652) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077942.1. Other_ESTs: H3020G07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3020 row: G column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..652 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3020G07-5" /db_xref="taxon:10090" /clone="H3020G07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 211 a 122 c 121 g 198 t ORIGIN 1 tgaaggcagg aggatcagga gttctaggcc agcctgcgat acatagtaag ttttaagtca 61 gactttcatg acagcctacc ttaaaaaaaa agtaaaataa attaaatgaa aataaaacct 121 agtcaggata atcctaccaa atgttttact atagacaaca atgttgttta tagatagagc 181 aaaatgtttt tataatggga aaaagaaaaa aggctgccat gcgggctggg attaaaggtg 241 tgtgccacca ctgcccagcc cagtttttct atttctgatt tttgagtact ggtattatat 301 gccaccatgt ctgactaaga ctgaattttt ttttaagact atttggttaa atggagaaaa 361 gatctacttc gcatctgtac ttatatatgt aatttacatt tttatatttc tataatcatt 421 ttagttactt ggaacctgtc caggatggga cactactata attccattac tcattatgag 481 cagagacagg aagaccttga actcatagac agccttagct acatagtaaa ccccatctca 541 aagtgaacag gctaggcctc tcaccttgca ttcgttccta ggtcatactt tagaactttc 601 ccaagcacag gaaggttagg aagccttcca ttctcatagc tcaggagagt ct //