LOCUS BG077899 600 bp mRNA linear EST 12-MAY-2010 DEFINITION H3020C06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3020C06 5', mRNA sequence. ACCESSION BG077899 VERSION BG077899.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077899.1. Other_ESTs: H3020C06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3020 row: C column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3020C06-5" /db_xref="taxon:10090" /clone="H3020C06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 155 c 175 g 107 t ORIGIN 1 ctgagcatca tccctgggtt accatgaacg acgtagccat tgtgaaggag ggctggctgc 61 acaaacgagg ggaatatatt aaaacctggc ggccacgcta cttcctcctc aagaacgatg 121 gcacctttat tggctacaag gaacggcctc aggatgtgga tcagcgagag tccccactca 181 acaacttctc agtggcacaa tgccagctga tgaagacaga gcggccaagg cccaacacct 241 ttatcatccg ctgcctgcag tggaccacag tcattgagcg caccttccat gtggaaacgc 301 ctgaggagcg ggaagaatgg gccaccgcca ttcagactgt ggcagatgga ctcaagaggc 361 aggaagaaga gacgatggac ttccgatcag gctcacccag tgacaactca ggggctgaag 421 agatggaggt gtccctggcc aagcccaagc accgtgtgac catgaacgag tttgagtacc 481 tgaagctact gggcaagggc acctttggga aggtgattct ggtgaaagag aaggccacag 541 gccgctacta tgccacgaag atcctcaaga aggaggtcat cgtcgccaag gatgaggttg //